Effects of Functionalized Carbon Nanotube on Cellular Homeostasis of Murine Hepatocytes

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Published: Oct 10, 2024
DOI: https://doi.org/10.47191/ijpbms/v4-i10-06
Keywords:
Carbon Nanotube; Nanoparticles; Cytotoxicity; Cellular viability; Oxidative stress; Murine hepatocytes.

Main Article Content

Krissia F. Godoy
Laboratory of Inflammation and Infectious Diseases, Federal University of São Carlos (UFSCar), 13565-905, São Carlos, SP, Brazil.
Joice M. A. Rodolpho
Laboratory of Inflammation and Infectious Diseases, Federal University of São Carlos (UFSCar), 13565-905, São Carlos, SP, Brazil.
Bruna D. L. Fragelli
Center for Development of Functional Materials, Federal University of São Carlos (UFSCar), 13565-905, São Carlos, SP, Brazil.
Marcelo Assis
Department of Biosciences, Federal University of São Paulo (UNIFESP). 11015-020. Santos, SP, Brazil.
Carlos Speglich
Leopoldo Américo Miguez de Mello Research Center CENPES/Petrobrás, Rio de Janeiro, Brazil
Elson Longo
Center for Development of Functional Materials, Federal University of São Carlos (UFSCar), 13565-905, São Carlos, SP, Brazil
Fernanda F. Anibal
Laboratory of Inflammation and Infectious Diseases, Federal University of São Carlos (UFSCar), 13565-905, São Carlos, SP, Brazil.

Abstract

Nanotechnology in medicine represents a revolution offering significant advancements in the diagnosis, treatment, and prevention of diseases. This technology enables the development of devices and materials at the nanometer scale, allowing precise interactions with biological systems at the molecular and cellular levels. Among various nanomaterials, carbon nanotubes (CNTs) stand out due to their unique physical, chemical and mechanical properties. Due to their high strength, electrical and thermal conductivity and large surface area, CNTs have a wide range of applications. In healthcare they are explored for drug delivery and imaging diagnostics. CNTs can be functionalized to target drugs directly to diseased cells, minimizing side effects and enhancing treatment efficacy, additionally can be used in biosensors for early disease detection and in tissue engineering to cellular regeneration. The present study evaluated the effects of OCNT-TEPA, a multi-walled carbon nanotube functionalized, in murine hepatocytes AML-12. Cells were exposed to different concentrations of the sample for 12, 24, 48, and 72 hours. Cellular metabolism tests, cellular morphology by optical microscopy, synthesis of reactive oxygen species, changes in membrane potential and IL-6 cytokine secretion were performed. The results show that OCNT-TEPA altered the homeostasis of hepatocytes, as cellular metabolism decreased, cellular morphology was altered, the membrane experienced changes in its electrical potential, oxidative stress increased and inflammatory signaling molecules were synthesized. This alteration is dose-dependent, which may be harmful to hepatocytes at high concentrations but could be applied to the body at lower concentrations without causing harm.

Article Details

How to Cite
Godoy, K. F., Joice M. A. Rodolpho, Bruna D. L. Fragelli, Marcelo Assis, Carlos Speglich, Elson Longo, & Fernanda F. Anibal. (2024). Effects of Functionalized Carbon Nanotube on Cellular Homeostasis of Murine Hepatocytes. International Journal of Pharmaceutical and Bio Medical Science, 4(10), 816–825. https://doi.org/10.47191/ijpbms/v4-i10-06
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Vol. 4 No. 10 (2024): Volume 04 Issue 10 October 2024
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This work is licensed under a Creative Commons Attribution 4.0 International License.

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