Testing the Inhibitory Activity of Bajakah Tampala (Spatholobus littoralis Hassk) Wood Extract from South Kalimantan on the Growth of Pseudomonas aeruginosa Bacteria
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Abstract
Background: Dental root canal treatment aims to eliminate microorganisms that cause infection in the tooth root canal, one of which is Pseudomonas aeruginosa bacteria. Disinfection of dental root canals with 0.5% - 5.25% NaOCl irrigation solution is still constrained so it is necessary to look for alternatives. The bajakah tampala plant (Spatholubus littoralis Hassk) is known to contain antibacterial compounds, namely flavonoids, saponins, tannins, and polyphenols.
Objective: To evaluate the inhibitory activity of bajakah tampala wood extract (Spatholobus littoralis Hassk) from South Kalimantan against the growth of Pseudomonas aeruginosa bacteria.
Method: Post test-only with control group design. The test groups were 100%, 75%, 50% bajakah tampala wood extract solution and positive control 2.5% NaOCL solution. Extraction using maceration method. Bacterial growth inhibition was tested by well diffusion method. Inhibition zone was measured using a caliper. Results: The growth inhibition from the largest to the smallest was shown by 75%, 100% and 50% bajakah wood extracts with mean inhibition zone diameters of 5.05 mm, 3.84 mm and 2.02 mm respectively, meanwhile the positive control was 16.14 mm. One Way Anova test showed significant difference in inhibition (p<0.05) in all groups. Post hoc test showed no significant difference in the inhibition of 75% bajakah tampala wood extract with 100% extract. The inhibition of 100% and 75% extracts was significantly greater than 50% extract. The inhibition of NaOCL 2.5% was significantly greater than all groups of test extracts.
Conclusion: Bajakah tampala wood extract has inhibitory activity against the growth of Pseudomonas aeruginosa bacteria.
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