Toxicity Test, Antioxidant Activity, and Determination of Total Flavonoid Content (TFC) of N-Hexane and Ethyl Acetate Fraction from Uncaria Nervosa Elmer (Bajakah) Root Wood
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Abstract
Uncaria nervosa Elmer (local name: Bajakah) is one of the medicinal plants used as a cancer drug by the people of Muara Badak, East Kalimantan. The purpose of this study was to determine the total flavonoid content (TFC), toxicity, and antioxidant activity of the n-hexane (NH) and ethyl acetate (EA) fractions of Uncaria nervosa root wood. Determination of total flavonoids was carried out using a UV-Vis spectrophotometer with quercetin as a standard, toxicity test was carried out using the brine shrimp lethality test (BSLT) method, and antioxidant test using the DPPH radical reduction method. Based on the results of this study, the total flavonoid content (TFC) of NH and EA fractions were 192 and 425 mg QE/g extract, respectively. Based on the results of the toxicity test on Artemia salina L. shrimp, the LC50 values were 11.91 ppm for NH and 33.19 ppm for EA. These LC50 values indicate that NH is very toxic and EA is toxic against Artemia salina L. While the antioxidant test results showed that EA was very significant as an antioxidant and NH was moderate with IC50 values of 17.54 ppm and 75.93, respectively.
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