Characterization of Pathogenic Bacteria Associated with Contamination of Domestic Water in Owerri Area
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Abstract
This study examined characterization of bacteria pathogens associated with domestic water contamination in Owerri area. Ten samples of water were collected from various sources of water in Owerri and were bacteriologically analyzed. Using the Pour-plate method on nutrient agar, MacConkey, and Salmonella-Shigella agar and the Most Probable Number technique, the total heterotrophic count and the most probable number index of coliform using double and single strength MacConkey broth as well as confirmed and completed test were determined. Result was presented in mean ± standard deviation, and Log10cfu/ml. The total heterotrophic count ranges from 7.8 ± 1.06 x 105cfu/ml to 6.1 ± 0.21 x 105cfu/ml, total coliform count ranges from 4.0 ± 1.41 x 104 cfu/ml to 1.5 ± 0.0 x 104cfu/ml. The Log10cfu/ml for heterotrophic count ranges from 8.7 Log10cfu/ml to 5.79 Log10cfu/ml, total coliform ranges 3.6 Log10cfu/ml to 1.9 Log10cfu/ml. Student t - test was used in comparing the mean between total heterotrophic count and total coliform count. The result revealed that there is a significant difference between the mean P > 0.005. Escherichia coli, Klebsiella species, Staphylococcus aureus, Pseudomonas species, Salmonella species and Enterobacter species were the organisms identified. Water is indispensable for human health and wellbeing. Portable water is an essential amenity that will aid eradication of water-borne diseases as well as improve the environmental sanitation. There should be enlightenment programs for the communities and other concerned populace in order to educate them on the dangers of such water sources serving for drinking or utilization for other domestic activities
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