Characterization of Proteus Spp. And Plasmid Mediated Resistance Among Hospitalized Patients in Eku, Delta State, Nigeria
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Abstract
The study was conducted to analyze the occurrence and antimicrobial susceptibility profile of Proteus species in blood and wound samples from patients who visited Eku General Hospital in Delta State, Nigeria. A total of 60 samples, 30 from blood and 30 from wound, were collected for the study. The bacteria were identified through bacteriological and biochemical analyses, while antibiotic susceptibility testing was performed using the Kirby-Bauer disc diffusion method. Plasmid curing was carried out using a sub-inhibitory concentration of 10% sodium dodecyl sulfate on resistant organisms. The study found three isolates including Proteus mirabilis, P. vulgaris, and P. penneri. The results showed that P. mirabilis was the most prevalent in blood (32.3%) and wound (22.6) samples, followed by P. penneri (11.3%), and P. vulgaris (9.7%) in blood sample. The study also found that the highest rate of isolation of the bacterium occurred among individuals aged between 21-35 years, followed by 12-20 years. Result of antibiotic susceptibility test showed high level of resistance in the two most commonly prescribed antibiotics cephalosporins and ampicillin. No resistance was observed in gentamycin making it the best drug for treating empirical infections. However, resistance was mostly carried on plasmids as shown by the loss of resistance after curing by the isolates. Harbouring of resistance on plasmids as huge public health implications including but not limited to spread of resistance genes, as such measures should be put in place to prevent spread.
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References
I. Akortha, R E. and Egbule, 0.S. (2008). Transfer of tetracycline gene (tet1) Between Replicons in some enteric bacteria of Diarrhoeal origin from some Hospitals in the South-South Nigeria. African Journal of Biotechnology,7(18), 3178-3181
II. Egbule, O. S. Owhe-Ureghe, U. B. and Odih E. E. (2016a). Occurrence of multidrug resistance among E.coli 0157: H7 isolated from stool samples obtained from Hospitalized children. Journal of probiotics and Health 4: 3: 1-4 Indexed by EBSCO, DRJI, Google scholar., (International).
III. Egbule, O. S. (2016). Antimicrobial Resistance and β-Lactamase Production among Hospital Dumpsite Isolates. Journal of Environmental protection 7 (07), 1057-1063
IV. Egbule, O. S. and Yusuf Ibrahim (2019). Multiple antibiotic Resistance in Escherichia coli isolated from cattle and poultry faeces in Abraka, south- south Nigeria. Tropical Agricultural Science. 42(2) 585-594. Indexed by Scopus (International)
V. *Iweriebor, B.C., Egbule, O.S., Igwaran, A. and Obi, L.C. (2021). Seogroups and Antibiogram of Salmonella Isolates from dairy cattle in Nkonkobe District, South Africa. Malaysian Journal of Microbiology, 174(4): 2021. Indexed by Scopus
VI. Iweriebor, B.C , Egbule, O.S and Obi, L.C (2022). The Emergence of Colistin-and Imipenem-Associated Multidrug Resistance in Isolates from Retail Meat. Polish Journal of Microbiology 71 (4), 519-528
VII. Egbule, OS (2022). Occurrence of extended spectrum beta–lactamases and sul 1 in multi-drug resistant Escherichia coli and Salmonella isolated from poultry feeds. Scientific African 18, e01362
VIII. Hatfull GF, Dedrick RM, Schooley RT. Phage therapy for antibiotic-resistant bacterial infections. Ann Rev Med. 2022;73:197-211.
IX. Szabo O, Gulyas D, Szabo N, Kristof K, Kocsis B, Szabo D. Plasmid-mediated quinolone resistance determinants in Enterobacteriaceae from urine clinical samples. Acta Microbiol Immunol Hung. 2018;65(3):255-265.
X. Kwiecińska-Piróg J, Skowron K, Zniszczol K, Gospodarek E. The assessment of Proteus mirabilis susceptibility to ceftazidime and ciprofloxacin and the impact of these antibiotics at subinhibitory concentrations on Proteus mirabilis biofilms. Biomed Res Int. 2013;2013:930876. doi: 10.1155/2013/930876. Epub 2013 Sep 12. PMID: 24151628; PMCID: PMC3787586.
XI. Facciolà A, Gioffrè M.E , Chiera D , Ferlazzo M, Virga A , Laganà P (2022). Evaluation of antibiotic resistance in Proteus spp: a growing trend that worries Public Health. Results of 10 Years of Analysis. New Microbiologica, 45, 4, 269-277, ISSN 1121-7138
XII. Girlich D., Bonnin R.A., Dortet L., Naas T. (2020). Genetics of Ac quired Antibiotic Resistance Genes in Proteus spp. Frontiers in Microbiology. 11, 256.
XIII. Adeolu, M., Gupta, R. S., Alnajar, S., and Naushad, S. Genome-based phylogeny and taxonomy of the "Enterobacteriales": Proposal for Enterobacterales ord. nov., subdivided into the families Enterobacteriaceae, Yersiniaceae fam. nov., Yersiniaceae fam. nov., Hafniaceae fam. nov., Morganellaceae fam. nov., and Budviciaceae fam. nov. Int. J. Syst. Evol. Microbiol. 66, 5575–5599 (2016).
XIV. Mobley, H. L. T., Pearson, M. M., and Armbruster, C. E. P. mirabilis infection pathogenesis. 2018; EcoSal Plus 8, 1–123.
XV. Armbruster CE, Mobley HLT. 2012. Combining morphology and mythology: Proteus mirabilis's varied way of life. 10:743–754 in Nat Rev Microbiol.
XVI. Foris LA, Snowden J, Jamil RT. Proteus mirabilis Infections. [Updated 2023 Jun 12]. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2024 Jan-. Available from: https://www.ncbi.nlm.nih.gov/books/NBK442017/
XVII. Drzewiecka, D. Significance and roles of Proteus bacteria in natural environments. Microb. Ecol. 72, 741–758 (2016
XVIII. Pearson MM, Schaffer JN. Proteus mirabilis and urinary tract infections. Microbiol Spectr. 2015;3(5):UTI-0017-2013. doi: 10.1128/microbiolspec.UTI-0017-2013 [PMC free article]
XIX. Jamil RT, Foris LA, and Snowden J. Proteus mirabilis infections. Treasure Island, FL, USA: StatPearls, 2019.
XX. C. Y. Lin, Y. H. Chen, P. L. Lu, W. R. Lin, and C.-Y. Chen, T. C. Chen. Risk factors, clinical presentation, and consequences of Proteus mirabilis urinary tract infection and bacteremia 2012, 45, 228 in J Microbiol Immunol Infect.
XXI. Infectious Diseases Society of America; Hooton TM, Rice JC, Saint S, Schaeffer AJ, Tambayh PA, Tenke P, Nicolle LE; Bradley SF, Cardenas DD, Colgan R, Rice JC, and Geerlings SE. The Infectious Diseases Society of America's 2009 International Clinical Practice Guidelines cover the diagnosis, prevention, and treatment of adult urinary tract infections linked to catheter use. Epub 2010 M
XXII. Luzzaro F, Perilli M, Amicosante G, et al. Properties of multidrug-resistant, ESBL-producing Proteus mirabilis isolates and the possible role of β-lactam/β-lactamase inhibitor combinations. Int J Antimicrob Agents. 2001;17(2):131–135. doi: 10.1016/S0924-8579(00)00325-3.
XXIII. Yang Q., Zhang H., Cheng J., et al. In vitro activity of flomoxef and comparators against Escherichia coli, Klebsiella pneumoniae, and proteus mirabilis-producing extended-spectrum β-lactamases in China. Int J Antimicrob Agents. 2015;45(5):485–490. doi: 10.1016/j.ijantimicag.2014.11.012.
XXIV. Empel J., Baraniak A., Literacka E., et al. Molecular survey of β-lactamases conferring resistance to newer β-lactams in Enterobacteriaceae isolates from Polish hospitals. Antimicrob Agen Chem. 2008;52(7):2449–2454. doi: 10.1128/AAC.00043-08
XXV. Laura J. V. Piddock, Maria Laura Ciusa, and Michelle M. C. Buckner. Strategies to counteract antimicrobial resistance are plasmid curing and anti-plasmid. Nov. 2018, FEMS Microbiol Rev. 42(6): 781–804. 30 July 2018; doi: 10.1093/femsre/fuy031. PMCID: PMC6199537; PMID: 30085063.
XXVI. Maheshwari DR, Sharma DR, Bachhiwal DR, and Mahima D. Antibacterial susceptibility pattern among proteus species isolated in a tertiary care hospital from different clinical samples. 2018 Sch J App Med Sci;6:1909–1913.
XXVII. Alhaj NA, Alsafani MS, Bahaj SS. Hospital acquired extended-spectrum beta-lactamase producing species: prevalence and possible risk factors. J Med Microbiol Open Access 2019;09:139–50.
XXVIII. Hakim FT, Gress, RE. Immuno-senescence: Adaptive immunity deficiencies in the aged. Tissue Antigens, 70 (3), 179–189, 2007. condition
XXIX. Omole A. and Stephen E. Antibiogram profile of bacteria isolated from patients' wound infections in three Anyigba, Kogi State, Nigerian hospitals. Journal of Res Sci. FUTA, 2014;(2):258–266.
XXX. Nawaz I, Zafar A, Taj M K, Taj I, Zafar U. Description of Proteus mirabilis Isolated from Patient Wounds at the Bolan Medical Complex Hospital, Quetta. J Microbiol Jundishapur. 2019;12(7):e87963. The DOI is 10.5812/jjm.87963.