Subtype Determination of Blastocystis Spp. Isolated from Poultry Tikrit City
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Abstract
Blastocystis spp. spread throughout the world, it is one of the intestinal parasites that settle in human’s gastrointestinal tract, as well as occurring in other hosts such as Aves, reptiles, cattle and insects. Gross stool samples collected from poultry were 221 samples. Blastocystis spp. and subtypes investigated by a PCR reaction which targeting the small subunit of ribosomal RNA (SSU rRNA) gene. Microscopic results and sequencing analysis results of SSU rRNA gene showed 2 sample were infected with Blastocystis with percentage 0.9% out of 221 poultry fecal samples. The poultry infected with Blastocystis consisted of chicken and turkey, as infected rate in chicken were 0.6% out of 163 chickens while infected rate in turkey were 1.7% out of 58 turkeys. The existence of subtype (ST6) of Blastocystis was confirmed via BLAST analysis.
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