HPTLC Fingerprint Profile and Standardization of Plantago ovata Forssk.

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Published: Jan 29, 2024
DOI: https://doi.org/10.47191/ijpbms/v4-i1-06
Keywords:
Plantago ovata, Phytochemical, HPTLC profiling, Microbial activity, Physicochemical evaluation, Microscopical evaluation

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Ananya M
PES University, 100ft Ring Road, BSK 3rd Stage, Dwaraka Nagar, Bengaluru, Karnataka, India
Nikhita Mirekar
PES University, 100ft Ring Road, BSK 3rd Stage, Dwaraka Nagar, Bengaluru, Karnataka, India
Sana Iddalagi
Vriksha Vijnan Private Limited, 31/2, SSB Complex, Subramanyapura Main Road Bengaluru, Karnataka, India
Narayanachar
Associate Professor, Department of Chemistry, LVD College Raichur, Karnataka, India
Vijay Danapur
Vriksha Vijnan Private Limited, 31/2, SSB Complex, Subramanyapura Main Road Bengaluru, Karnataka, India

Abstract

Plantago ovata belongs to the family Plantaginaceae and has notable medicinal properties as it is used for curing diarrhoea, dysentery and constipation due to its property of absorbing and retaining high amount of water. Methanolic extracts of P. ovata were obtained through reflux extraction to perform further experiments. Various experimental tests were carried out to understand the physicochemical, antimicrobial and fluorescence properties. HPTLC and phytochemical profiles were also obtained. Preliminary phytochemical analysis showed the presence of alkaloids, saponins, steroids and triterpenes. Microscopy of the powdered sample revealed the presence of starch granules, trichome and mucilage. The maximum antimicrobial activity of P. ovata was seen at a sample concentration of 10 µL where the activity was found to be more than standard against Bacillus cereus and Aspergillus niger. HPTLC profiling showed 9 bands each in 5 lanes of increasing concentration and a pattern unique to Plantago ovata. 

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How to Cite
Ananya M, Nikhita Mirekar, Sana Iddalagi, Narayanachar, & Vijay Danapur. (2024). HPTLC Fingerprint Profile and Standardization of Plantago ovata Forssk. International Journal of Pharmaceutical and Bio Medical Science, 4(1), 37–43. https://doi.org/10.47191/ijpbms/v4-i1-06
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Vol. 4 No. 1 (2024): Volume 04 Issue 01 January 2024
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Articles
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This work is licensed under a Creative Commons Attribution 4.0 International License.

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