A Study of Methanol Seed Extract of Lepidium Sativum Effect on Some Entero Bacteria and Fungi (Asparagillus Niger)

Main Article Content

Melath K. Al-Ghufaili
Shurooq F. Hassan
Shaymaa A. Abbas

Abstract

The results of the current study indicated the effect of methanol seed extract at a concentration of 200mg/ml there was a variation effect of L. sativum methanol seed extract against the studied bacteria, in the diameter of the inhibition zone with diver’s bacterial strain Staph. aureus gave the highest inhibition zone value was 26mm at 1ml of L. sativum methanol seed extract while E. coli and E. faecelis did not give any inhibition zone at 0.12 ml of L. sativum methanol seed extract, there was a variation of effect among gram-negative and positive bacteria in their response to different solvent seed extracts.


There was a variation effect in the antifungal activity of the methanol seed extract against Aspergillus niger the results established the methanol seed extract appeared the differences in their antioxidant activity and diverse variety. It was variation in the activity in relation to concentration (activity decrease with decrease extract concentration), Seed extract showed antioxidant activity in this study, the anti-oxidation effect of the L. sativum seeds extract by methanol, which give the highest percentages at concentration 1mg, reaching 91.419 and the lowest value at concentration 0.12, which amounted to 81.308. The present study deals with, antimicrobial properties and Anti-oxidant activity constituents of Gc seeds.

Article Details

How to Cite
Melath K. Al-Ghufaili, Shurooq F. Hassan, & Shaymaa A. Abbas. (2023). A Study of Methanol Seed Extract of Lepidium Sativum Effect on Some Entero Bacteria and Fungi (Asparagillus Niger). International Journal of Pharmaceutical and Bio Medical Science, 3(12), 709–715. https://doi.org/10.47191/ijpbms/v3-i12-02
Section
Articles

References

I. Agarwal N. and Sharma S (2013). Exploration and utilization of garden cress seeds (Lepidium sativum) through product development endeavor”. In: Gaur, R. K.; Sharma, K. P.; Chundawat RS (ed) Microbial, Plant and Animal Research. pp. 69–74.

II. Alqahtani F Y, Aleanizy F S, Mahmoud A Z, et al. (2019). Chemical composition and antimicrobial, antioxidant, and anti-inflammatory activities of Lepidium sativum seed oil. Saudi J. Biol. Sci. 26(5):1089–1092

III. AL-Saadi T R M N (2018). Molecular identification of some maize genotypes using EST-SSR and ISSR markers”, MSc thesis. University of kufa. Faculty of science. Iraq.96.

IV. Amarowicz R R B, Pegg P (2004). Rahimi-Moghad(dam; B. Barl; J.A. Weil.“Free-radical scavenging capacity and antioxidant activity of selected plant species from the Canadian prairies, Food Chemistry 84, 551–562.

V. Angel R and Chadha YR (1979). The Wealth of India. Raw Materials. Kew Bull, 32:802.

VI. Arabia H W A A, Saud (2010). Protective Effect of Lepidium sativum L. Seeds Powder and Extract on Hypercholesterolemic Rats. J. Am. Sci. 6(11):873–879.

VII. Chen, Xi, Yuan H, Shi Fand Zhu Y (2019). Effect of Garden Cress in Reducing Blood Glucose, Improving Blood Lipids and Reducing Oxidative Stress in a Mouse Model of Diabetes Induced by a High Fat Diet and Streptozotocin. J. Sci. Food Agric, 100:2074–2081.

VIII. Chopra R, Nayar S, Chopra I (2006).Glossary of Indian Medicinal Plants. 1956. Epub ahead of print1956.DOI: 10.1016/j.gyobfe.11.001.

IX. Egorove, N S (1985). Antibiotics Scientific Approach. Mirpublishers. Moscow.

X. El-Sayed S A , Bazaid S A , Shohayeb M M , El-Sayed M M and El- Wakil, E A (2012).Phytochemical Studies and Evaluation of Antioxidant, Anticancer and Antimicrobial Properties of Conocarpus erectus L. Growing in Taif, Saudi Arabia. European Journal of Medicinal Plants. 2(2): 93-112.

XI. Endrise J , Assefa Z , Dawo F , Abera D (2019).An in vitro antibacterial activity test of Phytolaca dodecandra, Adhatodaschimperiana and lepidium sativum against two major skin bacterial pathogens of equine. Glob. Vet. 21(4):232–239.

XII. Flora of North America, http://www.efloras.org/florataxon.aspx? flora_id=1&taxon_id=200009589, reviwed on 14-4-2020. Nature 1972; 239:414–414.

XIII. Gemede H F, Haki G D, Woldegiorgis F A F and Rakshit S K (2019). Phenolic profiles and antioxidant activities of ethiopian indigenousokra (Abelmoschus esculentus) pod and seed accessions: a new source of natural antioxidants. Annals. Food Science and Technology. 20(4): 809-819.

XIV. Gursoy, N, Sarikurkcu, C, Tepe, B, and Solak, M H (2010). Evaluation of antioxidant activities of 3 edible mushrooms: Ramaria flava (Schaef. Fr.) Quél. Rhizopogon roseolus (Corda) TM Fries., and Russula delica Fr, Food Science and Biotechnology, 19(3):691-696.

XV. Ishimaru K , Nishikawa K , Omoto T , Asai I ,Yoshihira K and Shimomura K (1995). Two flavone 2-glucosides from Scutellaria baicalensis, Phytochemistry, 40 (1): 279–281.

XVI. Jain T and Grover K (2018). A Comprehensive Review on the Nutritional and Nutraceutical Aspects of Garden Cress (Lepidium sativum Linn.) Proc. Natl. Acad. Sci. India Sect. B - Biol. Sci. 88(3):829–836.

XVII. Jangra S S and Madan V.K (2018). Phytochemical and Nutritional Composition of Different Parts of Garden Cress (Lepidium sativum L.) Int. J. Curr. Microbiol. Appl. Sci. 7(11):1136–1145.

XVIII. Kluytmans W J A J, Overdevest A M T L, Willsensen I and Bergh (2013). Extended-Spectrum β-lactamase- producing Escherichai coli from Retail Chicken Meat and Human: Comparison of Strains, Plasmids, Resistance Genes, and Virulence factors. Clinical infectious Disease. 56(4):478-487

XIX. Maltas E , Vural H C and Yildiz S (2011).Antioxidant activity and fatty acid composition of Ginkgo biloba from Turkey”. Food Biochem, 35:803-818.

XX. Maltas E and Yildiz S (2012). Evaluation of Phytochemicals and antioxidant activity of Ginkgo biloba from Turkey. Pharmacologia, 3(4):113-120

XXI. Mohamed D A , Abdelgayed S S and Essa HA, et al. (2018).Preparation and evaluation of functional foods for prevention of non-alcoholic fatty liver disease". Pakistan J. Biol. Sci. 21(9):454–462.

XXII. Mohamed D A, Essa H A and Mohamed R S (2019).Purslane and garden cress seeds as source of unconventional edible oils for prevention of hyperlipidemia. Pakistan J. Biol. Sci., 22(11):537–544.

XXIII. Noshirvani N and Fasihi H (2018). Control of Aspergilus niger in vitro and in vivo by three Iranian essential oils”, International Food Research Journal 25(4): 1745-1752

XXIV. Owusu-Ansah, M (2010). Investigations into propagation and characterisation of Moringa (Moringa oleifer

XXV. LAMARK)”, MphiL thesis, Graduate School of Nuclear and Allied Sciences, University of Ghana, 124-133.

XXVI. Pawar V and Thaker V S (2006). In vitro efficacy of 75 essential oils against Aspergillus niger,mycoses,49(4):316-23.

XXVII. Pellegrini N , Ying M and Rice C (1999). Evans Screening of dietary carotenoids and carotenoid-rich fruits extract for antioxidant activities applying 2, 2-azobis (3-ethylbenzothine-6-surfonic acid) radical cation decolorization assay,” Methods in Enzymology,vol. 299, pp. 384–389.

XXVIII. Region Q, Arabia S and Al-harbi K B et al (2016). The antidiarrheal activity of the methanol extract of some plants native to Al-Qassim Region, Saudi Arabia. J. food Agric. Environ. 14(2):238–243.

XXIX. Richard, A H, Pamela C C and Bruce, C F (2007). Microbiology, 2nd.p:69, 84, 91.

XXX. Saeid v, Parya k, Mahna K, Sajjad V E and Michael R Hamblin (2022). Anti-inflammatory and Immunomodulatory Properties of Lepidium sativum , BioMed Research International, Article ID 3645038, 12 pages, https://doi.org/10.1155/2022/3645038

XXXI. Sakran M, Selim Y, Zidan N (2014).A new isoflavonoid from seeds of Lepidium sativum L. and its protective effect on hepatotoxicity induced by paracetamol in male rats. Molecules. Epub ahead of print,

DOI: 10.3390/molecules191015440.

XXXII. Shah M B, Dudhat V A and Gadhvi K V (2021).Lepidium sativum: A potential functional food, Journal of Ayurvedic and Herbal Medicine, 7(2): 140-149.

XXXIII. Sharma M, Arora A and Gupta S (2018). Ethno-medicinal studies of plants used for the treatment of asthma by indigenous communities of chamba (himachal pradesh). Int. J. Pharm. Biol. Sci., 8(1):314–317.

XXXIV. Shivapratap H R, Philip T and Sharma D D (1996).The species concept in Fusarium .Indian journal of Seri,35(2):107-110.

XXXV. Tounsi N, Djerdjouri B and Yahia O A et al. (2019). Pro-oxidant versus anti-oxidant effects of seeds aglycone extracts of Lepidium sativum and Eruca vesicaria Linn. in vitro, and on neutrophil nitro-oxidative functions . J. Food Sci. Technol, 56(12):5492–5499.

XXXVI. Ullah M A, Tungmunnithum D and Garros L, et al (2019). Effect of ultraviolet-C radiation and melatonin stress on biosynthesis of antioxidant and antidiabetic metabolites produced in in vitro callus cultures of lepidium sativum L. Int. J. Mol. Sci. Epub ahead of print. DOI: 10.3390/ijms20071787.

XXXVII. Wu D T , Niey, X R , Sheny D D , Li H Y , Zhao L , Zhang Q , Lin D R and Qin, W, (2020).Phenolic compounds, antioxidant activities and inhibitory Eects on digestive enzymes of different cultivars of okra (Abelmoschus esculentus)”. Molecules, 25: 1276.